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Sar1 Activation Assay Kit
2023-06-08
产品详情
- 产地 China
- 价格:6800
- 产品名称Sar1 Activation Assay Kit
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- 简介
Kit Components
1. Anti active Sar1, Mouse Monoclonal Antibody (Catalog No. 26916): One vial – 22 μL
(1mg/ml) in PBS, pH 7.4, containing 50% glycerol and 0.05% sodium azide. This antibody
specifically recognizes Sar1 GTP from all vertebrates.
2. Protein A/G Agarose (Catalog No. 30301): One vial – 400 μL of 50% slurry.
3. 5X Assay/Lysis Buffer (Catalog No. 30303): One bottle – 30 mL of 50 mM HEPES (pH8),
0.1mg/ml BSA ,0.1%Triton-100, 1 mM DTT.
4. Anti Sar1, Rabbit Polyclonal Antibody (Catalog No. 21090): One vial – 22 μL (1 mg/ml) in
PBS, pH 7.4, contained 50% glycerol.
5. 100 X GTPγS (Catalog No. 30302): One vial –100 μl at 10 mM, use 5 μL of GTPγS forGTP-
labeling
of 0.5 mL of cell lysate.
6. 100 X GDP (Catalog No. 30304): One vial –100 μl at 100 mM, use 5 μL of GDP for GDP-
labeling of 0.5 mL of cell lysate.
Storage
Store all kit components at 4oC until their expiration dates.
Materials Needed but Not Supplied
1. Stimulated and non-stimulated cell lysates
2. Protease inhibitors
3. 4 °C tube rocker or shaker
4. 0.5 M EDTA, pH8.0
5. 1 M MgCl2
6. 2X reducing SDS-PAGE sample buffer
7. Electrophoresis and immunoblotting systems
8. Immunoblotting wash buffer such as TBST (10 mM Tris-HCl, pH 7.4, 0.15 M NaCl, 0.05%
Tween-20)
9. Immunoblotting blocking buffer (TBST containing 5% Non-fat Dry Milk or 3% BSA)
10. PVDF or nitrocellulose membrane
11. Secondary Antibody
12. ECL Detection Reagents
Reagent Preparation
? 1X Assay/Lysis Buffer: Mix the 5X Stock briefly and dilute to 1X in deionized water. Just prior to
usage, add protease inhibitors such as 1 mM PMSF, 10 μg/mL leupeptin, and 10 μg/mL aprotinin.
Note:
For research use only .
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