产品展厅
产品展厅
YXX05201
Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 μl.
E. coli
Streptococcus pyogenes
EndoS is cloned from Streptococcus pyogenes and expressed in E.coli.
109.35 kDa
Recombinant
Endo S
40000U/ml
Please contact with the lab for this information.
>95% as determined by SDS-PAGE.
Expression Systems,Proteomics,Glycan Sequencing,Recombinant Glycoprotein Expression,Glycoprotein Analysis
Liquid
0.01M PBS, pH 7.4, 50% glycerol
Protocol
Digestion reaction condition
|
Reagent |
Volume |
|
Native IgG concentration(μg/μl) |
C |
|
Native IgG volume (μl) |
V |
|
Native IgG quality (μg) |
100 |
|
Endo S(U) |
20 |
|
Endo S(μl) |
0.5 |
|
10X buffer(μl） |
200 |
|
ddH2O |
(200*10)-0.5-V |
|
Reaction system(μl) |
200*10 |
Incubate reaction at 37°C for 1 hour.
1X Buffer
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
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