产品展厅
产品展厅
关键信息(Key Features):
|
Full Name |
Cyclic adenosine monophosphate |
|
Synonym |
Cyclic adenosine monophosphate;cAMP;cyclic AMP;3'-5'-cyclic adenosine monophosphate; |
|
Assay Type |
Competitive |
|
Reactivity |
Universal |
|
Range |
1.56-100ng/mL |
|
Sensitivity |
0.93ng/mL |
|
Sample Type |
Serum,plasma and other biological fluids |
|
Sample volume |
50μL |
|
UNIProt ID |
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Microplate |
96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle)：
本试剂盒采用竞争ELISA法。用cAMP抗原包被于酶标板上，实验时样品或标准品中的cAMP与包被的cAMP竞争生物素标记的抗cAMP单抗上的结合位点，游离的成分被洗去。加入HRP酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，cAMP浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中cAMP的浓度。
存储(Storage)：
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
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典型数据(Typical Data): |
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cAMP ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
