产品展厅
产品展厅
关键信息(Key Features):
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Full Name |
Folic Acid/Vitamin B9 |
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Synonym |
FA, pteroyl-L-glutamic acid, vitamin B9, vitamin Bc, vitamin M, folacin, pteroyl-L-glutamate |
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Assay Type |
Competitive |
|
Reactivity |
Universal |
|
Range |
1.56-100ng/mL |
|
Sensitivity |
0.93ng/mL |
|
Sample Type |
Serum,plasma and other biological fluids |
|
Sample volume |
50μL |
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UNIProt ID |
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Microplate |
96-wells plate breakable into 12 x 8 wells strip |
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反应原理(Test Principle)： 本试剂盒采用竞争ELISA法。用FA/VB9抗原包被于酶标板上，实验时样品或标准品中的FA/VB9与包被的FA/VB9竞争生物素标记的抗FA/VB9单抗上的结合位点，游离的成分被洗去。加入HRP酶标记的亲和素，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，FA/VB9浓度与OD450值之间呈反比，通过绘制标准曲线计算出样品中FA/VB9的浓度。
存储(Storage)： Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. |
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典型数据(Typical Data): |
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FA/VB9 ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
