产品展厅
产品展厅
关键信息(Key Features):
|
Full Name |
Vascular Endothelial Cell Growth Factor A |
|
Synonym |
VEGF-A;VEGFA;MVCD1;VPF; |
|
Assay Type |
Sandwich |
|
Reactivity |
Mouse |
|
Range |
31.25-2000pg/mL |
|
Sensitivity |
18.75pg/mL |
|
Sample Type |
Serum,plasma and other biological fluids |
|
Sample volume |
100μL |
|
UNIProt ID |
Q00731 |
|
Microplate |
96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle)：
本试产品采用双抗体夹心ELISA法。用抗Mouse VEGF-A抗体包被于酶标板上，实验时样品（或标准品）中的Mouse VEGF-A会与包被抗体结合。后依次加入生物素化的抗Mouse VEGF-A抗体和HRP酶标记的亲和素，抗Mouse VEGF-A抗体与结合在包被抗体上的Mouse VEGF-A结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Mouse VEGF-A浓度与OD450 OD值之间呈正比，通过绘制标准曲线计算出样品中Mouse VEGF-A的浓度。
存储(Storage)：
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
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典型数据(Typical Data): |
|
|
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Mouse VEGF-A ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
