关键信息(Key Features):
Full Name |
Neutrophil Gelatinase Associated Lipocalin |
Synonym |
LCN2;Lipocalin 2;Oncogene 24p3;MSFI; |
Assay Type |
Sandwich |
Reactivity |
Mouse |
Range |
39.06-2500pg/mL |
Sensitivity |
23.43pg/mL |
Sample Type |
Serum,plasma and other biological fluids |
Sample volume |
100μL |
UNIProt ID |
P11672 |
Microplate |
96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle): 本试产品采用双抗体夹心ELISA法。用抗Mouse NGAL抗体包被于酶标板上,实验时样品(或标准品)中的Mouse NGAL会与包被抗体结合。后依次加入生物素化的抗Mouse NGAL抗体和HRP酶标记的亲和素,抗Mouse NGAL抗体与结合在包被抗体上的Mouse NGAL结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Mouse NGAL浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Mouse NGAL的浓度。
存储(Storage): Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions. |
典型数据(Typical Data): |
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Mouse NGAL ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |