关键信息(Key Features):
Full Name |
intercellular adhesion molecule 1 |
Synonym |
CD54 |
Assay Type |
Sandwich |
Reactivity |
Human |
Range |
0.31-20ng/mL |
Sensitivity |
0.18ng/mL |
Sample Type |
Serum,plasma and other biological fluids |
Sample volume |
100μL |
UNIProt ID |
P05362 |
Microplate |
96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle): 本试产品采用双抗体夹心ELISA法。用抗Human ICAM-1/CD54抗体包被于酶标板上,实验时样品(或标准品)中的Human ICAM-1/CD54会与包被抗体结合。后依次加入生物素化的抗Human ICAM-1/CD54抗体和HRP酶标记的亲和素,抗Human ICAM-1/CD54抗体与结合在包被抗体上的Human ICAM-1/CD54结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在HRP酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,Human ICAM-1/CD54浓度与OD450 OD值之间呈正比,通过绘制标准曲线计算出样品中Human ICAM-1/CD54的浓度。
存储(Storage): Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
|