产品展厅
产品展厅
关键信息(Key Features):
|
Full Name |
Leptin |
|
Synonym |
LEPD;OBS;Obesity Homolog; |
|
Assay Type |
Sandwich |
|
Reactivity |
Human |
|
Range |
15.62-1000pg/mL |
|
Sensitivity |
9.37pg/mL |
|
Sample Type |
Serum,plasma and other biological fluids |
|
Sample volume |
100μL |
|
UNIProt ID |
P41159 |
|
Microplate |
96-wells plate breakable into 12 x 8 wells strip |
反应原理(Test Principle)：
本试产品采用双抗体夹心ELISA法。用抗Human LEP抗体包被于酶标板上，实验时样品（或标准品）中的Human LEP会与包被抗体结合。后依次加入生物素化的抗Human LEP抗体和HRP酶标记的亲和素，抗Human LEP抗体与结合在包被抗体上的Human LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Human LEP浓度与OD450 OD值之间呈正比，通过绘制标准曲线计算出样品中Human LEP的浓度。
存储(Storage)：
Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.
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典型数据(Typical Data): |
|
|
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Human LEP ELISA Standard Curve Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting. |
