关键信息(Key Features):

反应原理(Test Principle)：

本试产品采用双抗体夹心ELISA法。用抗Human TNF-α抗体包被于酶标板上，实验时样品（或标准品）中的Human TNF-α会与包被抗体结合。后依次加入生物素化的抗Human TNF-α抗体和HRP酶标记的亲和素，抗Human TNF-α抗体与结合在包被抗体上的Human TNF-α结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在HRP酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，Human TNF-α浓度与OD450 OD值之间呈正比，通过绘制标准曲线计算出样品中Human TNF-α的浓度。

存储(Storage)：

Store the product at 2-8°C before unopened. Upon receipt, unpack promptly and store as recommended in the instructions.

典型数据(Typical Data):

Human TNF-α ELISA Standard Curve

Typical data are for reference only and curves should be replotted for each experiment. The Logistics function is recommended for fitting.