11.SDS-PAGE

Determined by SDS-PAGE, the purity of the protein is greater than 95%.

12.SEC-HPLC

Determined by SEC-HPLC, the purity of the protein is greater than 95%.

When Recombinant Human HER3(Ser20-Thr643)/His Protein is immobilized at 2μg/mL (100 μL/well), the concentration of Anti-Her3 [patritumab] that produces 50% of the optimal binding response is approximately 100 ng/ml.