11.SDS-PAGE
Determined by SDS-PAGE, the purity of the protein is greater than 95%.
12.SEC-HPLC
Determined by SEC-HPLC, the purity of the protein is greater than 95%.
13.ELISA
When Recombinant Human HER2/hFC Protein is immobilized at 2μg/mL (100 μL/well), the concentration of Pertuzumab (Cat.No. B162801.abinvivo) that produces 50% of the optimal binding response is approximately 9 ng/ml.
14.SPR
Anti-Human HER2 (Pertuzumab)(Cat.No.B162801)captured on CM5 chip , can bind Recombinant Human HER2/hFC Protein(Cat.No.B152702)with a KD constant of 8.15E-11 M, determined in a SPR assay .
15.Note:For research use only .