1.Alternative Name:Roundabout homolog 4
2.Target:Robo4
3.Source:Human Robo4 was expressed from HEK293 with poly His at the C-terminal
4.Sequence:Gln28-Glu469
5.Protein structure:Q8WZ75His
6.Molecular Characterization:The protein has a calculated MW of 49kDa.The reducing (R) heterodimer protein migrates as 50-80 kDa due to glycosylation
7.Purity:SDS-PAGE>95%,SEC>95%
8.Reconstitution:sterile H2O
9.Endotoxin:<1EU/mg
10.Formulation:Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
11.SDS-PAGE
Determined by SDS-PAGE, the purity of the protein is greater than 95%.
12.SEC-HPLC
Determined by SEC-HPLC, the purity of the protein is greater than 90%.
12.ELISA
When Recombinant Human Robo4/His Protein is immobilized at 5μg/mL (100 μL/well), the concentration of Anti Human Robo4 antibody that produces 50% of the optimal binding response is approximately 80 ng/ml.
13.Note:For research use only .
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