11.SDS-PAGE

Determined by SDS-PAGE, the purity of the protein is greater than 95%.

Determined by SEC-HPLC, the purity of the protein is greater than 95%.

13.ELISA

When Recombinant Human CD95/hFC Protein is immobilized at 2μg/mL (100 μL/well), the concentration of Anti Human-FAS Antibody(clone h-HFE7A) that produces 50% of the optimal binding response is approximately 40 ng/ml.

Recombinant Human CD95/hFC Protein（Cat.No.B21815602）captured on Protein A chip , can bind Recombinant Human CD178(134-281)/His Protein（Cat.No.B21736902） with a KD constant of 1.38E-09 M, determined in a SPR assay .