1.Alternative Name:TNFSF7,CD27LG;CD27 Ligand; CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
2.Target:CD70
3.Source:Human CD70 was expressed from HEK293 with human IgG1 FC at the C-terminal
4.Sequence:Ser52-Pro193
5.Protein structure::P32970hFC
6.Molecular Characterization:The protein has a calculated MW of 73kDa.The non-reducing (N-R) heterodimer protein migrates as 140-160 kDa due to glycosylation
7.Purity:SDS-PAGE>95%,SEC>90%
8.Reconstitution:sterile H2O
9.Endotoxin:<1EU/mg
10Formulation:Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
11.SDS-PAGE
Determined by SDS-PAGE, the purity of the protein is greater than 95%.
12.SEC-HPLC
Determined by SEC-HPLC, the purity of the protein is greater than 90%.
13.ELISA
When Recombinant Human CD70/ hFC Protein is immobilized at 5μg/mL (100 μL/well), the concentration of Anti-Human CD70 (Cusatuzumab) that produces 50% of the optimal binding response is approximately 20 ng/ml.
14.SPR
Recombinant Human CD70/ hFC Protein(Cat.No.B22045006)captured on Protein A chip , can bind Recombinant Human CD27/ His Protein(Cat.No.B22045002) with a KD constant of 4.95E-07M, determined in a SPR assay .
15.Note:For Research Use Only.
...