1.Alternative Name:Fc gamma RIIB/C,FCGR2B/C;CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b
2.Target:CD32b
3.Source:Human CD32b was expressed from HEK293 with poly His at the C-terminal
4.Sequence:Ala46-Pro217
5.Protein structure:P26842hFC
6.Molecular Characterization:The protein has a calculated MW of 21kDa.The reducing (R) heterodimer protein migrates as 30-40 kDa due to glycosylation
7.Purity:SDS-PAGE>95%,SEC>95%
8.Reconstitution:sterile H2O
9.Endotoxin:<1EU/mg
10.Formulation:Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
Note:For research use only .
11.SDS-PAGE
Determined by SDS-PAGE, the purity of the protein is greater than 95%.
12.SEC-HPLC
Determined by SEC-HPLC, the purity of the protein is greater than 95%.
13.SPR
Recombinant Human IgG1 /hFC Protein(Cat.No.B1591)captured on Protein A chip , can bind Recombinant Human FC-gamma RII-b(CD32b)/His Protein(Cat.No.B22249601)with a KD constant of 2.69E-06 M, determined in a SPR assay .
14.SPR
Anti-Human HER2(Margetuximab) (Cat.No.B623802)captured on Protein A chip , can bind Recombinant Human FC-gamma RII-b(CD32b)/His Protein(Cat.No.B22249601)with a KD constant of 8.78E-06 M, determined in a SPR assay .
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