1.Alternative Name:LEU3;CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
2.Target:CD4
3.Source:Human CD4 was expressed from HEK293 with poly His at the C-terminal
4.Sequence:Lys26-Gln390
5.Protein structure:P01730His
6.Molecular Characterization:The protein has a calculated MW of 42kDa.The reducing (R) heterodimer protein migrates as 40-60 kDa due to glycosylation
7.Purity:SDS-PAGE>95%,SEC>95%
8.Reconstitution:sterile H2O
9.Endotoxin:<1EU/mg
10.Formulation:Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
Note:For research use only .
11.SDS-PAGE
Determined by SDS-PAGE, the purity of the protein is greater than 95%.
12.SEC-HPLC
Determined by SEC-HPLC, the purity of the protein is greater than 95%.
13.ELISA
When Recombinant Human CD4/His Protein is immobilized at 2μg/mL (100 μL/well), the concentration of Recombinant Anti-Human CD4 (Ibalizumab) that produces 50% of the optimal binding response is approximately 70 ng/ml.
14.SPR
Recombinant Anti-Human CD4 (Ibalizumab)(Cat.No.B554401)captured on Protein A chip , can bind Recombinant Human CD4/His Protein(Cat.No.B21656903)with a KD constant of 2.42E-11 M, determined in a SPR assay .
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