Introduction:
The Chemi - Trans™ Lipofectin Transfection Reagent is a proprietary lipid
based transfection reagent that forms a complex with DNA or small RNA,
and can efficiently deliver the complex into a broad range of commonly used
mammalian cell lines. Chemi - Trans™ Lipofectin Transfection Reagent has
been proven to get a good performance as well as Lipofectamine® 2000
Reagent in DNA and siRNA transfection,
Advantages:
? Good transfection efficiency and low cytotoxicity.
? Provides a convenient transfection for both plasmid DNA and small
RNA.
? Compatible with serum and antibiotics in culture medium.
Important Guidelines for Transfection:
(1) For maximum transfection efficiency, using serum-free medium (such as
Opti-MEM® Reduced Serum Medium) to dilute DNA is a must.
(2) While the standard protocols for DNA transfection being given below,
optimization is often needed for maximal transfection efficiency.
For Research Use Only. Not for use in diagnostic procedures.
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