Introduction:
The Chemi-Trans™ Polyfectin DNA Transfection Reagent is formulated as a
biodegradable polymer-based DNA delivery tool that performs well for DNA
transfecting in a variety of commonly used and hard-to-transfect mammalian
cell lines. A remarkable feature of Chemi-Trans™ Polyfectin Reagent is the
rapid and complete degradation of polymer after transfection complex by
endocytosis, leading to much less cytotoxicity.
Advantages:
? Bio-degradable and low cytotoxicity.
? Excellent for long DNA transfection.
? Easy to use, forms a DNA complex in just five minutes.
Important Guidelines for Transfection:
(1) For maximum transfection efficiency, using serum-free medium (such as
serum-free DMEM with High Glucose. DO NOT use Opti-MEM Medium,
it contains serum and will disrupt transfection complex) to dilute Chemi -
Trans™ Polyfectin Reagent and DNA is a must.
(2) While the standard protocols for DNA transfection being given below,
optimization is often needed for maximal transfection efficiency.
For Research Use Only. Not for use in diagnostic procedures.
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