产品名称
骨桥素(OPN)活性蛋白
Active Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
物种：Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
缓冲液：成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
性状：冻干粉
纯度：> 95%
等电点：4.3
应用：Cell culture; Activity Assays.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
活性试验
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappaB pathways in MCF-7 breast cancer cell line. To test the bioactivity of recombinant rat OPN, MCF-7 cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of recombinant rat OPN for 48h and MCP-1 levels in the cell supernatant were determined by ELISA (SEA087Hu). MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN which was shown in Figure1, the EC50 was 9.83 ug/ml.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
Note
For research use only .
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