| Gene Symbol: RHOA |
| Description: Anti RhoA Rabbit Polyclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction. |
| Immunogen: A synthetic peptide from the C-terminal region of RhoA. |
| Applications: ELISA, WB, IHC, IP |
|
Recommended Dilutions: ELISA: 1:100-1:5000 WB: 1:100-1:2000 IHC: 1:10-1:1000 |
| Concentration: 0.2 mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 51% glycerol Species |
| Species Reactivity: RhoA antibody recognizes RhoA from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
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Note: |
Western blot analysis of RhoA proteins. Purified recombinant His-RhoA protein was used for western blot with the anti RhoA rabbit polyclonal antibody (Cat. # 21017). |