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atalog Number: CST26337
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Gene Symbol: NFE2L2, NRF2
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Description: Anti NFE2L2(E79K) Mouse Monoclonal Antibody
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Background: NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress.
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Immunogen: A synthetic peptide from the internal region of NFE2L2 which includes the mutation of E79K, human origin.
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Applications: ELISA, WB, IHC
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Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
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Concentration: 1 mg/ml
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Host: Mouse
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Format: Liquid
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Clonality: Monoclonal
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Isotype:
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Purity: Purified from ascites
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Storage buffer:
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Preservative: no
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Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
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Species Reactivity: Recognizes E79K mutant, but not wild-type NFE2L2 of vertebrates.
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Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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Western blot:
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Western blot analysis of recombinant NFE2L2(E79K) and wildtype proteins. Purified His-tagged NFE2L2(E79K) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti NFE2L2(E79K) monoclonal antibody (Cat. #26337).
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Immunofluorescence of cells expressing NFE2L2 proteins with Anti NFE2L2(E79K) antibody. HEK293T cells were transfected with pCDNA3-GFP-NFE2L2(E79K) plasmid, pCDNA3-GFP NFE2L2(WT) plasmid, or pCDNA3-GFP NFE2L2(E79Q) plasmid, then fixed and stained with Anti NFE2L2(E79K) monoclonal antibody (Cat. #26337).
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Note:
For research use only .
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