产品描述

Favipiravir (T-705), an effective and selective RNA-dependent RNA polymerase inhibitor, are applied to treat influenza virus infections.

靶点活性

RNA-dependent RNA polymerase

体外活性

Favipiravir shows anti-influenza virus activities with IC50 ranged from 0.013 to 0.48 μg/ml for the influenza A viruses, from 0.039 to 0.089 μg/ml for the influenza B viruses, and from 0.030 to 0.057 μg/ml for the influenza C viruses. In mammalian cell lines (MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells), Favipiravir shows no cytotoxicity at concentrations up to 1,000 μg/ml. [1] In MDCK cells inoculated with seasonal influenza A (H1N1) viruses, Favipiravir induces lethal mutagenesis. [2]

体内活性

In influenza virus-infected mice, Favipiravir (200 mg/kg/day, p.o.) protects the mice from death from influenza virus infection. [1] In mice experimentally infected with Ebola virus, Favipiravir efficiently blocks viral production, reaching an antiviral effectiveness of 95% and 99.6% at 2 and 6days after initiation of treatment, respectively. [3]

细胞实验

The cytotoxicity of T-705 is evaluated by an assay with XTT. XTT is converted to aqueous formazan by an enzyme in MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells. The compounds are diluted to the appropriate concentrations (volume, 100 μl) with test medium (EMEM containing 10% FCS) in 96-well culture plates in which each well contains a concentration of 2 × 103 cells/100 μL. The test plates are incubated for 3 days at 37°C in 100% humidity and 5% CO2. After 3 days, 50 μl of the XTT reagent (1 mg/ml in FCS-free EMEM containing 5 mM phenazine methosulfate) is added, and the reaction product is assayed by measurement of the absorbance at 450 nm with a microplate reader. Cytotoxicity is expressed as the 50% cell-inhibitory concentration (CC50).(Only for Reference) Cell lines: MDCK cells, Vero cells, HEL cells, A549 cells, HeLa cells, and HEp-2 cells

别名

法维拉韦 , 6-Fluoro-3-oxo-3,4-dihydropyrazine-2-carboxamide , T-705 , favilavir , 法匹拉韦 , 法维拉韦 , 6-Fluoro-3-oxo-3,4-dihydropyrazine-2-carboxamide , T-705 法匹拉韦
纯度

≥95%
分子量

157.1
分子式

C₅H₄FN₃O₂
CAS No.

259793-96-9

存储
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
溶解度
DMSO: 29 mg/mL (184.6 mM)
Ethanol: 12 mg/mL (76.4 mM)
Water: 5 mg/mL (31.82 mM), warmed
( < 1 mg/ml refers to the product slightly soluble or insoluble )
参考文献
4. WEI X U, Shuai X, Jing P, et al. The antihistamine drugs carbinoxamine maleate and chlorpheniramine maleate exhibit potent antiviral activity against a broad spectrum of influenza viruses[J]. Frontiers in Microbiology. 2018 Nov 6;9:2643.

5. Qiu M, Li Z, Chen Y, et al. Tolcapone Potently Inhibits Seminal Amyloid Fibrils Formation and Blocks Entry of Ebola Pseudoviruses. Frontiers in Microbiology. 2020, 11: 504.

Note

For research use only.