产品展厅
产品展厅
FITC-xtra 异硫氰酸荧光素是美国AAT Bioquest生产的荧光素 ，虽然FITC是用于制备绿色荧光生物共轭物的最流行的荧光标记染料，但是FITC存在某些限制，例如用于显微镜成像的严重光漂白和pH敏感荧光。与相应的FITC缀合物相比，用FITC-xtra制备的蛋白质缀合物是非常优秀的。FITC-xtra缀合物比FITC缀合物明显更亮，并且更加光稳定。另外，FITC-xtra的荧光不受pH（4-10）的影响。这种pH不敏感性是对FITC的重大改进，FITC仅在pH大于9时才发出其最大荧光，FITC-xtra具有几乎相同FITC的光谱特性。此外，FITC-xtra在温和缀合条件下比FITC产生高得多的缀合产率。像5-FITC，FITC-xtra抗体缀合物适合于488nm激光线的激发，使其成为相应的FITC标记的抗体缀合物的替代物。在检测条件相同的情况下，FITC-xtra抗体缀合物提供比相应的FITC标记的缀合物高得多的信号/背景比。
实验方案
1.准备蛋白质储备溶液（溶液A）：
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