产品名称：AF532 PEG4 生物素偶联物
规格：1mg
储存条件：保存在冰箱-15℃干燥
保质期：12个月
产品物理化学光谱特性
分子量：1172.48
外观：固体
溶剂：水
激发波长(nm)：534
发射波长(nm)：553
产品介绍
AF532 PEG4 生物素偶联物含有 Alexa Fluor 532 荧光团，可被常见的 532 nm 激光很好地激发。它在亲和素和链霉亲和素四聚体的高亲和力、快速结合和非协同结合方面具有与天然生物素相似的亲和素结合特性。 AF532 PEG4 生物素偶联物可用于检测和量化抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白的生物素结合位点。该试剂克服了常用的 Biotin-4-fluorescein 的主要缺点，例如在水性介质中的溶解度差和 pH 依赖性吸光度和短波长发射。 AF532 PEG4 生物素偶联物是一种水溶性试剂，具有从 pH 4 到 pH 10 的不依赖 pH 的荧光。生物素部分和荧光标签之间的柔性 PEG4 间隔基可最大限度地减少与抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白结合所涉及的空间位阻。
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