产品介绍

用于活细胞细胞表面染色的一些染料标记的荧光抗体缀合物通常在单核细胞和巨噬细胞中表现出非特异性结合。CytoWatch QZ100单核细胞封闭剂是一种非基于抗体的封闭溶液，经过优化可封闭基于花青的染料缀合物的非特异性背景。它可以有效地消除花青染料缀合物（例如PE / Cy5，PE / Cy7，APC / Cy7，PE / Dazzle 594，APC / Fire 750，PE / AlexaFluor®647， PE / AlexaFluor®750，APC / AlexaFluor®750，PE /iFluor®647，PE /iFluor®750和APC /iFluor®750）。该试剂对活淋巴细胞，单核细胞和粒细胞的特定表面染色没有影响。

实验方案

流式细胞仪分析的细胞表面染色方案

1.向100 μL PBMC或全血中加入5 μL CytoWatch QZ100单核细胞封闭试剂。
2.在室温下孵育5-10分钟或立即添加一抗，然后在室温下孵育20分钟。
3.用细胞染色缓冲液洗涤两次。
4.将细胞重悬于0.5 mL细胞染色缓冲液中。
5.进行流式细胞仪分析。

图示

图1.未经处理（左）或用CytoWatch QZ100单核细胞封闭剂（右）处理人外周血，并用CD3（克隆UCHT1）PE /Cy5染色。

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