适用仪器

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流式细胞仪
激发：	488nm激光
发射：	530/30 nm滤波片
通道：	FITC滤波片组

荧光显微镜
激发：	FITC滤波片
发射：	FITC滤波片
推荐孔板：	黑色透明

样品实验方案

以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。

简要概述

1. 根据需要处理样品
2. 准备Xite Green β-D-吡喃半乳糖苷工作溶液并将其添加到样品中
3. 在37°C下孵育样品15至45分钟
4. 使用带有530/30 nm滤光片的流式细胞仪（FITC通道）或带有FITC滤光片组的荧光显微镜检测荧光强度

溶液配制

储备溶液配制

操作步骤

1. 根据需要处理样品。
2. 处理并用自备的缓冲液（例如DPBS）洗涤细胞。
3. 加入Xite Green β-D-吡喃半乳糖苷工作溶液15-45分钟，然后在37°C的培养箱中培养样品。
   注意：孵育的最佳时间需要通过实验确定。
4. 取出工作溶液并用自备的缓冲液洗涤细胞。
5. 将细胞重悬在自备的缓冲液中，并使用流式细胞仪使用530/30 nm滤光片（FITC通道）或带有FITC滤光片组的荧光显微镜检测荧光强度。

图示

图1.用Xite Green β-D-吡喃半乳糖苷测量β-gal的表达。将9L-LacZ细胞（过度表达β-gal的细胞）与Xite Green β-D-半乳糖吡喃糖苷或FDG在37°C孵育30分钟。使用NovoCyte流式细胞仪（ACEA Biosciences）通过FITC通道获取信号。

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