产品名称：MMP Green 基质金属蛋白酶绿色荧光底物

规格：100 Tests

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：~2000

溶剂：DMF

激发波长(nm)：494

发射波长(nm)：515

产品介绍

基质金属蛋白酶（MMPs）是一个锌依赖性内肽酶家族，在细胞外基质中发挥作用。这些酶在负责结缔组织的分解，在骨重建、月经周期和组织损伤修复中起重要作用。虽然MMPs在某些病理过程中的确切作用尚不清楚，但MMPs似乎在关节炎以及肿瘤的侵袭和转移中起着关键作用。基质金属蛋白酶往往有多种基质，大多数家族成员都有能力降解不同类型的胶原蛋白以及弹性蛋白、明胶和纤连蛋白。很难找到对单一MMP酶有选择性的底物。该FRET底物被设计用于检测MMP酶的一般活性。当使用纯化的MMP酶时，它也可用于筛选MMP抑制剂。该FRET底物基于我们的tf/TQ2，MMP Green FRET由于tfi/TQ2 FRET对分离，FRET肽底物增加。荧光增强与MMP酶活性成正比。

图示

图1.内部淬火的FRET肽底物被蛋白酶消化以产生高荧光肽片段。荧光增强与蛋白酶活性成正比。

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