产品展厅
产品展厅
Xite Red β-D-半乳糖吡喃糖苷是β-半乳糖苷酶（β-gal）的荧光底物。与现有的β-半乳糖苷酶底物（例如，常用的FDG）相比，它具有更好的细胞通透性。Xite Red β-D-吡喃半乳糖苷很容易进入细胞，并被β-gal裂解，从而产生强荧光产品Xite Red。强烈荧光的Xite Red可以很好地保留在细胞中，从而易于通过流式细胞仪和荧光显微镜进行检测。Xite Red β-D-半乳糖吡喃糖苷提供了一种简单而灵敏的工具来检测β-半乳糖苷酶的活性。Xite Red β-D-吡喃半乳糖苷可以作为检测细胞中细胞衰老的工具，因为β-gal已被确定为细胞衰老的可靠标记。此外，Xite Red β-D-吡喃半乳糖苷是可固定的。Xite Red β-D-吡喃半乳糖苷产生的红色荧光可以很容易地与其他颜色的荧光探针（例如DAPI或GFP）结合使用，以进行多色荧光分析。
适用仪器
| 流式细胞仪 | |
| 激发： | 488nm激光 |
| 发射： | 575/26 nm滤波片 |
| 通道： | PE滤波片组 |
| 荧光显微镜 | |
| 激发： | Cy3/TRITC滤波片 |
| 发射： | Cy3/TRITC滤波片 |
| 推荐孔板： | 黑色透明 |
样品实验方案
以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。
简要概述
溶液配制
储备溶液配制
操作步骤
图示
图1.用Xite Red β-D-吡喃半乳糖苷测量β-gal的表达。将9L-LacZ细胞（过度表达β-gal的细胞）与Xite Red β-D-吡喃半乳糖苷在37°C下孵育30分钟。使用NovoCyte流式细胞仪（ACEA Biosciences）通过PE通道获取信号。 |
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