适用仪器

样品实验方案

储备溶液

ProLite His Tag 蛋白凝胶染色原液
在 ProLite His Tag 蛋白凝胶染料小瓶中加入 60 μL DMSO 制成储备溶液。
注意：1 瓶原液适用于 2 块凝胶。 将未使用的储备溶液储存在 -20°C。

工作溶液

ProLite His Tag 蛋白凝胶染色工作溶液

在 30 mL PBS 中加入 30 μL XdU 储备溶液，制成 ProLite His Tag 蛋白凝胶染色工作溶液。
注意：制备足够的工作溶液，使凝胶完全浸入工作溶液中。
注意：不要重复使用工作溶液。

实验步骤

A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.
Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
Journal: Chemical science (2020): 9648-9654

Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair.
Authors: Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M
Journal: The Journal of biological chemistry (2020): 12214-12223

The surface syndecan protein from Macrobrachium rosenbergii could function as mediator in bacterial infections.
Authors: Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong
Journal: Fish & shellfish immunology (2020): 62-68

[Cloning and expression of SmDXS2 gene in Swertia mussotii].
Authors: Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941

Biochemical and Functional Characterization of Mouse Mammary Tumor Virus Full-Length Pr77Gag Expressed in Prokaryotic and Eukaryotic Cells.
Authors: Chameettachal, Akhil and Pillai, Vineeta Narayana and Ali, Lizna Mohamed and Pitchai, Fathima Nuzra Nagoor and Ardah, Mustafa Taleb and Mustafa, Farah and Marquet, Roland and Rizvi, Tahir Aziz
Journal: Viruses (2018)

Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles.
Authors: Torres-González, Lisa and Díaz-Ayala, Ramonita and Vega-Olivencia, Carmen A and López-Garriga, Juan
Journal: Sensors (Basel, Switzerland) (2018)

Molecular characterization of Babesia microti thioredoxin (BmTrx2) and its expression patterns induced by antiprotozoal drugs.
Authors: Huang, Jingwei and Xiong, Kang and Zhang, Houshuang and Zhao, Yanzhen and Cao, Jie and Gong, Haiyan and Zhou, Yongzhi and Zhou, Jinlin
Journal: Parasites & vectors (2018): 38

Overexpression, Purification and Functional Characterisation of Wild-Type HIV-1 Subtype C Protease and Two Variants Using a Thioredoxin and His-Tag Protein Fusion System.
Authors: Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien
Journal: The protein journal (2018): 369-379

Facile fabrication of nickel immobilized on magnetic nanoparticles as an efficient affinity adsorbent for purification of his-tagged protein.
Authors: Rashid, Zahra and Naeimi, Hossein and Zarnani, Amir-Hassan and Mohammadi, Fereshteh and Ghahremanzadeh, Ramin
Journal: Materials science & engineering. C, Materials for biological applications (2017): 670-676

Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368).
Authors: Zhao, Dongxin and Huang, Zhongxian
Journal: Bioinorganic chemistry and applications (2016): 8206854