对于许多免疫组织化学（IHC）应用，传统的酶促扩增程序足以实现足够的抗原检测。但是，有几个因素限制了这些程序的敏感性和实用性。酪酰胺信号放大（TSA）已被证明是一种特别通用且功能强大的酶放大技术，具有更高的测定灵敏度。TSA基于HRP在低浓度过氧化氢存在下将标记的含酪胺底物转化为可与HRP或附近的酪氨酸残基共价结合的氧化的高反应性自由基的能力。Power Styramide™信号放大（PSA™）是对TSA系统的最新升级，具有比相应TSA试剂高10至50倍的改进荧光信号。

样品分析方案

概述

细胞或组织培养

在封闭缓冲液中添加一抗

加入结合HRP的二抗

准备Styramide™（PSA）或Tyramide（TSA）工作溶液，并在室温下在细胞或组织中涂抹5-10分钟 

储备溶液配制

将100μLDMSO加入到iFluor™染料标记的Styramide™缀合物（例如＃45000）的小瓶中，制成100X Styramide™储备液。
注意：制成单次使用的等分试样，并将未使用的100X储备溶液在2-8°C的暗处存储，并避免重复冻融循环。

向90μL的ddH ₂ O中添加10μL的3％过氧化氢（组分B）。
注意：在使用当天准备新鲜的100X H ₂ O ₂溶液。

工作溶液配制

每1 mL PSA工作溶液需要1 mL TSA / PSA缓冲液（组分A），10μLStyramide™储备溶液和10μLH ₂ O ₂储备溶液。
注意：1 mL足够进行10次测试，基于每个盖玻片或96孔微孔板中每孔所需的100μLStyramide™工作溶液。
注意：Styramide™工作溶液必须在制备后2小时内使用，并避免直接暴露在光线下。

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