产品名称：噻唑橙

CAS：107091-89-4

规格：100mg

储存条件：保存在冰箱-20℃干燥

保质期：12个月

产品物理化学光谱特性

分子量：476.61

外观：红色

溶剂：DMSO

Ex(nm):512

Em(nm):533

产品介绍

噻唑橙是用于网织红细胞分析的荧光DNA结合染料。它对疟原虫物种分析也很有用。改性噻唑橙衍生物（如SYBR Green，PicoGreen，TOTO和YOYO）广泛用于检测DNA样品。

染色细胞示例

以下程序适用于大多数细胞类型。生长培养基，细胞密度，其他细胞类型的存在和其他因素可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的真实或明显染色，导致在有或没有细胞存在的溶液中出现明亮染色的物质。

1）制备1-10mM DMSO储备溶液。如果储存在-20°C，DMSO储备液可以保存6个月。

2）使用适合您样品的固定方案。

3）通过离心沉淀细胞，并将细胞重悬于缓冲盐溶液或培养基中，在pH 7.4下进行最佳染料染色。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。

4）使用0.5和10μM之间的浓度添加噻唑橙染色并孵育15至60分钟作为指导。

注意：在最初的实验中，最好在整个建议范围内尝试几种染料浓度确定产生最佳染色的浓度。

参考文献

Effect of UVC-Induced Photooxidation of DNA on the Intercalation of Thiazole Orange: A Convenient Reporter for Oxidative Damage
Authors: Trevithick-Sutton CC, Mikelsons L, Filippenko V, Scaiano JC.
Journal: Photochem Photobiol. (2006)

Exploring Base-Pair-Specific Optical Properties of the DNA Stain Thiazole Orange
Authors: Jarikote DV, Krebs N, Tannert S, Roder B, Seitz O.
Journal: Chemistry. (2006)

Fluorescence resonance energy transfer and complex formation between thiazole orange and various dye-DNA conjugates: implications in signaling nucleic acid hybridization
Authors: Algar WR, Massey M, Krull UJ.
Journal: J Fluoresc (2006): 555

Large dynamic Stokes shift of DNA intercalation dye Thiazole Orange has contribution from a high-frequency mode
Authors: Karunakaran V, Perez Lustres JL, Zhao L, Ernsting NP, Seitz O.
Journal: J Am Chem Soc (2006): 2954

Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties
Authors: Skripchenko A, Wagner SJ, Thompson-Montgomery D, Awatefe H.
Journal: Transfusion (2006): 213

Forced intercalation probes (FIT Probes): thiazole orange as a fluorescent base in peptide nucleic acids for homogeneous single-nucleotide-polymorphism detection
Authors: Kohler O, Jarikote DV, Seitz O.
Journal: Chembiochem (2005): 69

Sequence-specific fluorescence detection of DNA by polyamide-thiazole orange conjugates
Authors: Fechter EJ, Olenyuk B, Dervan PB.
Journal: J Am Chem Soc (2005): 16685

Synthesis and fluorescence studies of thiazole orange tethered onto oligonucleotide: development of a self-contained DNA biosensor on a fiber optic surface
Authors: Wang X, Krull UJ.
Journal: Bioorg Med Chem Lett (2005): 1725

Double staining of Plasmodium falciparum nucleic acids with hydroethidine and thiazole orange for cell cycle stage analysis by flow cytometry
Authors: Jouin H, Daher W, Khalife J, Ricard I, Puijalon OM, Capron M, Dive D.
Journal: Cytometry A (2004): 34

Thiazole orange positive platelets in a dog with Evans' syndrome
Authors: Michimoto T, Okamura T, Suzuki K, Watari T, Kano R, Hasegawa A.
Journal: J Vet Med Sci (2004): 1305