产品名称：Aminopropargyl ddCTP [5-Propargylamino-2',3'-dideoxycytidine-5'-triphosphate]

CAS：114748-56-0

规格：10 umoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：592.15

外观：液体

溶剂：DMF

激发波长(nm)：N/A

发射波长(nm)：N/A

产品介绍

Sanger法是DNA测序中最早 的方法之一，DNA由四种脱氧核苷酸三磷酸（dNTP）合成。将每个新核苷酸添加到 的dNTP的3'-OH基团中。可以将二脱氧胸苷三磷酸（ddTTPs）添加到正在生长的DNA链中，但是当它出现时，链延长会停止，因为下一个要连接的核苷酸没有3'-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP，dGTP，dCTP和dTTP的混合物。加入四种双脱氧核苷酸（ddATP，ddGTP，ddCTP和ddTTP）的混合物，每种混合物均以限量存在，并分别用发不同颜色的荧光的“标签”标记。因为所有四个正常核苷酸都存在，所以链延伸正常进行，直到偶然地DNA聚合酶插入ddNTP（而不是正常dNTP）。如果正常核苷酸与双脱氧形式的比率足够高，则在插入ddNTP之前，某些DNA链将成功添加数百个核苷酸，从而终止该过程。在孵育期结束时，片段的长度从最长到 分离，一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时，四个DDNTP中的每一个都会发出不同的荧光，可以通过自动扫描仪打印输出序列。这些ddATP，ddGTP，ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。

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