产品名称：Amplite ADHP过氧化物和过氧化酶荧光探针

规格：25mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：257.24

外观：白色固体

溶剂：DMSO

激发波长（nm）：571

发射波长（nm）：585

产品介绍

Amplite ADHP过氧化物和过氧化酶荧光探针是美国AAT Bioquest生产的过氧化氢酶荧光底物 ，Amplite ADHP与AmplexRed 在化学上是相同的（AmplexRed 是Invitrogen的商标）。它是一种敏感的荧光过氧化物酶底物，其背景比其他商业供应商的材料低得多。ADHP产生高荧光的试卤灵，其最大吸收为571nm，最大发射为585nm。与其他HRP底物如二氢荧光素和二氢罗丹明不同，ADHP的空气氧化是最小的。到目前为止，ADHP被认为是用于检测HRP和H₂O₂的最灵敏和最稳定的荧光探针。ADHP已被广泛用于检测许多免疫测定中的HRP。另一方面，ADHP也可用于检测痕量的H₂O₂。基于ADHP的H₂O₂检测比常用的硫氧还蛋白对H₂O₂的检测灵敏度至少高一个数量级。由于H₂O₂是在许多酶促氧化还原反应中产生的，因此ADHP可用于偶联酶促反应，以检测许多氧化酶和/或相关酶/底物或辅助因子如葡萄糖，乙酰胆碱和胆固醇，L-谷氨酸，氨基酸等的活性。简单地制备10-20mM DMSO储备溶液，然后稀释到测定缓冲液如PBS，Tris缓冲液中，用于测定50-200μM。

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